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Strep-tag II;   Strep-tag 

Mouse   Monoclonal Antibody 

IgG1

Protein A/G Purification

ELISA, WB, IP

Species independent

Peptide ASWSHPQFEKGA

PBS with 0.1% sodium azide and 50% glycerol,   pH 7.2 

Store at -20℃.   Do not aliquot

WB: 1:1000-2000         IP:   3ug/sample

The   Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK   ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a   specifically engineered streptavidin and can be N- or C- terminally fused to   recombinant protein. The Strep-tag II peptide was developed as an affinity   tool for the purification of corresponding fusion proteins on streptavidin   columns. The peptide recognizes the same pocket of streptavidin where the   natural ligand is normally bound so that biotin or its chemical derivatives   can be used for competitive elution. 

For research use only .