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CS26904

Anti Active RhoA Mouse Monoclonal Antibody

Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.

Recombinant full length protein of active RhoA

IP, IHC

1 mg/ml

mouse

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Liquid

Monoclonal

IgG2b

Purified from ascites

no

PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol

Anti-active RhoA antibody recognizes active RhoA from vertebrates.

     
IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins wer
e loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated
with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE,
membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).                                                                                                        
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Store at -20°C. Avoid freeze / thaw cycles

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For Research Use Only.