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一、重組抗體表達(dá)

重組抗體被廣泛地應(yīng)用在科學(xué)研究、免疫診斷及治療性抗體領(lǐng)域。科斯坦生物生物推出CASRATM保證型重 組抗體表達(dá)服務(wù)。科斯坦生物生物自有的哺乳表達(dá)系統(tǒng)高產(chǎn)量pATX系列載體及馴化293系列和CHO系列細(xì)胞， 能實(shí)現(xiàn)全長(zhǎng)抗體以及多種形式的重組抗體片段的高效分泌表達(dá)。無(wú)需免疫，可實(shí)現(xiàn)單克隆抗體及其它 形式重組抗體的高產(chǎn)和可重復(fù)生產(chǎn)。

二、服務(wù)內(nèi)容

CSRA^TM保證型重組抗體表達(dá)服務(wù)內(nèi)容

三、服務(wù)流程

四、武漢科斯坦生物優(yōu)勢(shì)

1.科斯坦生物擁有抗體藥物研發(fā)專(zhuān)家和技術(shù)支持團(tuán)隊(duì)，可對(duì)您感興趣的抗體講行個(gè)性化的序列優(yōu)化設(shè)計(jì)，提高重組抗體表達(dá)量。全長(zhǎng)抗體分泌表達(dá)量可達(dá)100-300mg/L實(shí)現(xiàn)一次表達(dá)終身受益。

2.豐富的抗體表達(dá)經(jīng)驗(yàn)和技術(shù)團(tuán)隊(duì)，已經(jīng)成功表達(dá)過(guò)多種形式的全長(zhǎng)抗體(HumanIgG1~4，Mous e IgG2b,MouseIgG2a,RabbitIgG等)抗體片段(scfv，F(xiàn)ab,tandem-scfv，VHH，F(xiàn)c融合蛋白。 bi-specificantibody等)。

3.科斯坦生物擁有多種服務(wù)平臺(tái)，在實(shí)現(xiàn)重組抗體設(shè)計(jì)表達(dá)和純化的同時(shí)，同時(shí)可以實(shí)現(xiàn)抗體的活性驗(yàn)證。目前已經(jīng)設(shè)計(jì)完成了多個(gè)嵌合抗體的表達(dá)及活性驗(yàn)證，并對(duì)部分人鼠嵌合抗體進(jìn)行人源化設(shè)計(jì)驗(yàn)證。真正實(shí)現(xiàn)一站式服務(wù)。

五、案例展示

Recombinant Antibody Production Cases

Expression caseI:scFv

Expression system:Bsubtilis ExpressionSystem

Service Type:Recombinant Protein Production

Conclusion: Total obtained 37mg scFy from 1L scale up purification, purity is about 90%.

Figure 1. Expression result

Figure 2. Target scFv purification profile

Figure 3. Final sample QC

Expression caseⅡ:Bi-specific Antibody

Expression System:Mammalian 293F Expression System

Service Type: Recombinant Antibody Production

Conclusion:Bi-specific antibody A total obtained 17.35mg per liter of culture, purity> 90% Bi-specific antibody B total obtained 16.23mg per liter of culture, purity> 85%.

Figure 2. Target protein purification profile

Figure 3. Final sample QC

Expression casellI: IgG-like bi-specific antibody

Expression System:Mammalian CHO-S Expression System

Service Type:Recombinant Antibody Production

Conclusion:Total obtained IgG-like: 104mg from 1L scale up purification, purity> 90%

Figure 1. Expression result

Figure 2. Target protein purification profile

Figure 3. Final sample QC

Expression caselII: HH expressed with tag and tag removal

Service Type:Recombinant Antibody Production

Conclusion: Total obtained VHH without any tag: 13.2mg from 1L scale up purification after tag removal, purity> 90%

Target protein purification profile in native condition.

Tag cleavage results.

1. Before cleavage sample 2. After cleavage sample

Final sample QC

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