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- 產(chǎn)品名稱： Gα13 Activation Assay Kit
- 產(chǎn)品貨號(hào)： 80401
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購： 6800
- 數(shù)量：
庫存： 5
- 規(guī)格： 96T
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Product Description
A structurally diverse repertoire of ligands, from photons to large peptides, activates GPCRs to elicittheir physiological functions. Ligand-bound GPCRs, in turn, function as guanine nucleotide exchangefactors catalyzing the exchange of GDP bound on the Gα subunit with GTP in the presence of Gβγ,causing the dissociation of the Gα subunit from the Gβγ dimer to form two functional units (Gα andGβγ). Both Gα and Gβγ subunits signal to various cellular signaling pathways. Based on the sequenceand functional homologies, G proteins are grouped into four families: Gs, Gi, Gq, and G12 . As increasingnumbers of effectors and interacting proteins for these G proteins have been identified, the physiologicalprocesses in which G proteins participate are multiplying.
Among the four subfamilies of G proteins, the function of G12/13 subfamily is less well understood. Inthis family, there are two members, G12 and G13, that are expressed ubiquitously. Gα12 knockout miceappeared normal. Gα13 knockout mice displayed embryonic lethality (~E9.5). The Gα13-/-mouseembryos had defective vascular systems. G13 is also essential for receptor tyrosine kinase-inducedmigration of fibroblast and endothelial cells. Bioyears Biosciences Gα13 Activation Assay Kit provides a simple and fast tool to monitor the
activation of Gα13. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences Gα13 Activation Assay Kit bases on the configuration-specific anti Gα13 GTPmonoclonal antibody to measure the active Gα13-GTP levels, either from cell extracts or from in vitroGTPγS loading Gα13 activation assays. Briefly, anti active Gα13 mouse monoclonal antibody will be incubated with cell lysates containing Gα13-GTP. The bound active Gα13 will then be pulled down byprotein A/G agarose. The precipitated active Gα13 will be detected by immunoblot analysis using antiGα13 rabbit polyclonal antibody.
Kit Components
1. Anti active Gα13, Mouse Monoclonal Antibody (Catalog No. 26902): One vial – 22 μL (1mg/ml) in PBS,
pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody specifically recognizes Gα13-GTP from all vertebrates.
pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody specifically recognizes Gα13-GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30303): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750mM NaCl, 5 mM EDTA, 5% Triton X-100.
3. 5X Assay/Lysis Buffer (Catalog No. 30303): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750mM NaCl, 5 mM EDTA, 5% Triton X-100.
4. Anti Gα13, Rabbit Polyclonal Antibody (Catalog No. 21005): One vial – 22 μL (1 mg/ml) in
PBS, pH 7.4, contained 50% glycerol.
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Storage
Store all kit components at 4oC until their expiration dates.
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Note
For Research Use Only.
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