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- 產(chǎn)品名稱： Anti SMO(A68V) Mouse Monoclonal Antibody
- 產(chǎn)品貨號： CST26425
- 貨期： 現(xiàn)貨
- 價格與訂購： 4800
- 數(shù)量：
庫存： 5
- 規(guī)格： 100μg
- 產(chǎn)品信息
- 如何訂購
Catalog Number
CST26425
Gene Symbol
SMO; MRD21
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Description
Anti SMO(A68V) Mouse Monoclonal Antibody
Background
moothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
Immunogen
A synthetic peptide from the internal region of SMO which includes the mutation of A68V, human origin.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes A68V mutant, but not wild-type SMO of vertebrates.
Immunoprecipitation/Western Blot
Western blot analysis of recombinant SMO(A68V) and wild-type proteins. Purified His-tagged SMO(A68V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti SMO(A68V) monoclonal antibody (Cat. #26425).
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Immunofluorescence:
Immunofluorescence of cells expressing SMO proteins with Anti SMO(A68V) antibody. HEK293T cells were transfected with pCDNA3-GFP SMO(WT) plasmid (left column) or pCDNA3-GFP SMO(A68V) plasmid (right column), then fixed and stained with Anti SMO(A68V) monoclonal antibody (Cat. #26425).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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