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CST26474

H-Ras; K-Ras; N-Ras

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Anti RAS(G13R) Mouse Monoclonal Antibody

 Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

 A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin.

ELISA, WB, IHC

1 mg/ml

mouse

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Liquid

Monoclonal

Purified from ascites

no

 PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Recognizes G13R mutant, but not wild-type RAS of vertebrates.

Western blot analysis of recombinant HRAS(G13R) and wildtype proteins. Purified His-tagged RAS(G13R) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with Anti RAS(G13R) monoclonal antibody (Cat. #26474).   
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Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13R) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid , pCDNA3-GFP Ras(G13C) plasmid , pCDNA3-GFP Ras(G13V) plasmid , pCDNA3-GFP Ras(G13A) plasmid , or pCDNA3-GFP Ras(G13D) plasmid , then fixed and stained with Anti RAS(G13R) monoclonal antibody (Cat. #26474).

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Store at -20°C. Avoid freeze / thaw cycles

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For Research Use Only.