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CST26909

RAS

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Anti Active Ras Mouse Monoclonal Antibody

 Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Recombinant full length protein of active H-Ras

IP, IHC

1 mg/ml

mouse

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Liquid

Monoclonal

IgG

Purified from ascites

no

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

nti active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.

                       IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti Ras mouse monoclonal antibody (Cat. # 26014).                             
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 Store at -20°C. Avoid freeze / thaw cycles

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For Research Use Only.