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- 產(chǎn)品名稱： Biotin DBCO
- 產(chǎn)品貨號(hào)： CSC330
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購(gòu)： 1800
- 數(shù)量：
庫(kù)存： 10
- 規(guī)格： 1 mg
- 產(chǎn)品信息
- 如何訂購(gòu)
Introduction
Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
The biotin DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC). Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin? biotin-binding protein.
Features
Efficiency—the click reaction is complete in less than 1 hour;
Specificity—the reaction between the label and detection tag is selective and specific;
Stability—the reaction product contains an irreversible, covalent bond;
Biologically inert—the components of the reaction do not undergo any side reactions.
Figure 1. Click chemistry labeling
Specifications
Label
Biotin
Ex/Em
–
Detection Method
–
Solubility
DMSO, DMF
Molecular Weight
747.95
Product Size
1 mg
Storage Conditions
-20 ℃, protect from light
Shipping Condition
Room Temperature
Applications
Click chemistry labeling
Reference
1.Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,Angew Chem Int Ed Engl (2012) 51:3143-3146
2.Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.
Winz ML, Samanta A, Benzinger D, J?schke A,Nucleic Acids Res (2012) 40:e78-e78
Note
For research use only .
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