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- 產(chǎn)品名稱： SMCC
- 產(chǎn)品貨號(hào)： CS00085
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購： 200
- 數(shù)量：
庫存： 10
- 規(guī)格： 50mg
- 產(chǎn)品信息
- 如何訂購
產(chǎn)品描述
N-Succinimidyl 4-(N-maleimidomethyl)cycl(SMCC), also known as succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate, is a heterobifunctional protein crosslinker.
體外活性
N-Succinimidyl 4-(N-maleimidomethyl)cycl（SMCC) is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.
細(xì)胞實(shí)驗(yàn)
Branched PEI (25 kDa) was dissolved in PBS buffer to yield a concentration of 1 mg/mL. No-weigh Sulfo-SMCC was dissolved in Milli-Q water with a concentration of 10 mg/mL, then a certain of the prepared no-weigh SulfoSMCC solution was added into 2 mL PEI solution to incubate for 30 min at 37 C. After the PEI-SMCC polymer was formed, TAT solution was added into the polymer at the given molar ratio and incubated for 2 h at 4 C. The concentration of unreacted SMCC and unreacted TAT were removed using Zeba Spin Desalting Columns.. The polymer PEI-SMCC-TAT was also characterized and analyzed through FTIR to check if each element was added to the PEI backbone.
別名
N-Succinimidyl 4-(N-maleimidomethyl)cycl , 4-(N-馬來酰亞胺基甲基)環(huán)己烷-1-羧酸琥珀酰亞胺酯 ,
純度
98%
分子量
334.32
分子式
C16H18N2O6
CAS No.
64987-85-5
存儲(chǔ)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
溶解度
DMSO: Soluble
( < 1 mg/ml refers to the product slightly soluble or insoluble )
參考文獻(xiàn)
1. Li F, Wang Z, Huang Y, Xu H, He L, Deng Yan, Zeng X, He N. Delivery of PUMA Apoptosis Gene Using Polyethyleneimine-SMCC-TAT/DNA Nanoparticles: Biophysical Characterization and In Vitro Transfection Into Malignant Melanoma Cells. J Biomed Nanotechnol. 2015 Oct;11(10):1776-82. PubMed PMID: 26502640.
2. Guo Y, et al. Potent antigen-specific immune response induced by infusion of spleen cells coupled with succinimidyl-4-(N-maleimidomethyl cyclohexane)-1-carboxylate (SMCC) conjugated antigens. Int Immunopharmacol. 2016 Feb;31:158-68.
Note
For research use only .
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